Phleum pratense (Common timothy) allergen 1
£200.00 – £1,500.00
Product name: Phleum pratense (Common timothy) allergen 1
Product description: Recombinant Phleum pratense (Common timothy) allergen 1 produced in Nicotiana benthamiana by Agrobacterium-mediated transient expression.
Host species: Nicotiana benthamiana
Molecular weight: 29.5 kDa
Tag: C-terminal His-tag
Uses: For in vitro research use only. Not for human in vivo or therapeutic use.
Relevance: Phleum pratense (Common timothy) Phl p 1 is the major allergen from grass pollen1. This glycoprotein is a group 1 allergen with a molecular mass of approximately 30 kDa2. Phl p 1 contains the majority of group 1–specific IgE and T cell epitopes3, and its homodimeric three-dimensional structure has been shown by x-ray crystallography. Phl p 1 is highly allergenic and can be used as a diagnostic marker to identify patients that are allergic to grass pollen due to its strong cross-reactivity with group 1 allergens from related grass species4,5.
Purity: 96 % as shown by SDS-PAGE
Figure 1: reducing 4-20% SDS-PAGE analysis of 5 µg Phl p 1.
Biological activity: Not tested.
Endotoxin: Not tested.
Formulation: Lyophilized from 50 mM HEPES pH7.5, 100 mM NaCl, 175 mM Trehalose Dihydrate.
Shipping: Recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Stability & Storage: See COA for detailed storage instructions. Lyophilized materials are stable for up to twelve months from date of receipt at -70℃.
It is recommended that reconstituted protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: A hardcopy of COA with reconstitution instructions is included with the product.
- Laffer S, Valenta R, Vrtala S, Susani M, van Ree R, Kraft D, Scheiner O, Duchêne M. Complementary DNA cloning of the major allergen Phl p I from timothy grass (Phleum pratense); recombinant Phl p I inhibits IgE binding to group I allergens from eight different grass species. J. Allergy Clin. Immunol. 94:689–698. (1994).
- Laffer S, Duchene M, Reimitzer I, Susani M, Mannhalter C, Kraft D, Valenta R. Common IgE-epitopes of recombinant Phl p I, the major timothy grass pollen allergen and natural group I grass pollen isoallergens. Mol. Immunol. 33:417–426. (1996).
- Bokanovic D, Aberer W, Hemmer W, Heinemann A, Komericki P, Scheffel J, Sturm GJ. Determination of sIgE to rPhl p 1 is sufficient to diagnose grass pollen allergy. Allergy. 68:1403–1409. (2013)
- Kahn CR, Marsh DG. Monoclonal antibodies to the major Lolium perenne (rye grass) pollen allergen Lol p I (Rye I) Mol. Immunol. 23:1281–1288. (1986)