Phleum pratense (Common timothy) allergen 1

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Description

Product name: Phleum pratense (Common timothy) allergen 1

Product description: Recombinant Phleum pratense (Common timothy) allergen 1 produced in Nicotiana benthamiana by Agrobacterium-mediated transient expression.

Description

Host species: Nicotiana benthamiana

Molecular weight: 29.5 kDa

Tag: C-terminal His-tag

Uses: For in vitro research use only. Not for human in vivo or therapeutic use.

Relevance: Phleum pratense (Common timothy) Phl p 1 is the major allergen from grass pollen1. This glycoprotein is a group 1 allergen with a molecular mass of approximately 30 kDa2.  Phl p 1 contains the majority of group 1–specific IgE and T cell epitopes3, and its homodimeric three-dimensional structure has been shown by x-ray crystallography. Phl p 1 is highly allergenic and can be used as a diagnostic marker to identify patients that are allergic to grass pollen due to its strong cross-reactivity with group 1 allergens from related grass species4,5.

Specification

Purity: 96 % as shown by SDS-PAGE

Figure 1: reducing 4-20% SDS-PAGE analysis of 5 µg Phl p 1.

 

Biological activity: Not tested.

Endotoxin: Not tested.

Storage

Formulation: Lyophilized from 50 mM HEPES pH7.5, 100 mM NaCl, 175 mM Trehalose Dihydrate.

Shipping: Recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.

Stability & Storage: See COA for detailed storage instructions. Lyophilized materials are stable for up to twelve months from date of receipt at -70℃.

It is recommended that reconstituted protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Reconstitution: A hardcopy of COA with reconstitution instructions is included with the product.

 

References

  1. Laffer S, Valenta R, Vrtala S, Susani M, van Ree R, Kraft D, Scheiner O, Duchêne M. Complementary DNA cloning of the major allergen Phl p I from timothy grass (Phleum pratense); recombinant Phl p I inhibits IgE binding to group I allergens from eight different grass species. J. Allergy Clin. Immunol. 94:689–698. (1994).
  2. https://www.uniprot.org/uniprot/P43213
  3. Laffer S, Duchene M, Reimitzer I, Susani M, Mannhalter C, Kraft D, Valenta R. Common IgE-epitopes of recombinant Phl p I, the major timothy grass pollen allergen and natural group I grass pollen isoallergens. Mol. Immunol. 33:417–426. (1996).
  4. Bokanovic D, Aberer W, Hemmer W, Heinemann A, Komericki P, Scheffel J, Sturm GJ. Determination of sIgE to rPhl p 1 is sufficient to diagnose grass pollen allergy. Allergy. 68:1403–1409. (2013)
  5. Kahn CR, Marsh DG. Monoclonal antibodies to the major Lolium perenne (rye grass) pollen allergen Lol p I (Rye I) Mol. Immunol. 23:1281–1288. (1986)