Human Nucleotide triphosphate diphosphatase NUDT15 (MTH2)

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Description

Product name: Human Nucleotide triphosphate diphosphatase NUDT15 (MTH2)

Product description: Recombinant Human Nucleotide triphosphate diphosphatase NUDT15 produced in Nicotiana benthamiana by Agrobacterium-mediated transient expression.

Description

Host species: Nicotiana benthamiana

Molecular weight: 19.4 kDa

Tag: C-terminal His-tag

Uses: For in vitro research use only. Not for human in vivo or therapeutic use.

Relevance: NUDT1 is a member of the NUDIX hydrolase superfamily of pyrophosphohydrolases that act upon nucleoside diphosphatates linked to other moieties, X1. NUDT15 is a 18.6 kDa dimeric, cytosolic enzyme2 that reportedly converts 8-oxo-dGTP and 8-oxo-dGDP to 8-oxo-dGMP leading to the assumption that it may contribute to sanitizing the oxidized dNTP pool3. Recent structural information has however indicated that NUDT15 prefers other nucleotide substrates over 8-oxo-dGTP and that cellular depletion of NUDT15 has no effect on incorporation of 8-oxo-dGTP into DNA and therefore does not impact cancer cell survival in cell lines tested4.

Specification

Purity: >95 % as shown by SDS-PAGE

Figure 1: reducing 4-20% SDS-PAGE analysis of 5 µg NUDT15.

Biological activity: Not tested.

Endotoxin: Not tested.

Storage

Formulation: Lyophilized from 20 mM HEPES pH8, 150 mM NaCl, 175 mM Trehalose Dihydrate.

Shipping: Recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.

Stability & Storage: See COA for detailed storage instructions. Lyophilized materials are stable for up to twelve months from date of receipt at -70℃.

It is recommended that reconstituted protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Reconstitution: A hardcopy of COA with reconstitution instructions is included with the product.

References

  1. Bessman M, Frick D & O’Handley, S. The MutT proteins or ‘Nudix’ hydrolases, a family of versatile, widely distributed, ‘housecleaning’ enzymes. J. Biol. Chem. 271, 25059–25062 (1996).
  2. https://www.uniprot.org/uniprot/Q9NV35
  3. Cai, J. P., Ishibashi, T., Takagi, Y., Hayakawa, H. & Sekiguchi, M. Mouse MTH2 protein which prevents mutations caused by 8-oxoguanine nucleotides. Biochem. Biophys. Res. Commun. 305, 1073–1077 (2003).
  4. Carter M, Jemth AS, Hagenkort A, Page BD, Gustafsson R, Griese JJ, Gad H, Valerie NC, Desroses M, Bostrom J, et al. Crystal structure, biochemical and cellular activities demonstrate separate functions of MTH1 and MTH2. Nat Commun.;6:7871. doi: 10.1038/ncomms8871. (2015).