Bet v 1-A – Betula Verrucosa (European white birch) Allergen 1-A


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Product name: Bet v 1-A – Betula verrucosa (European white birch) allergen 1-A

Product description: Recombinant Betula verrucosa (European white birch) allergen 1-A produced in Nicotiana benthamiana by Agrobacterium-mediated transient expression.



Host species: Nicotiana benthamiana

Molecular weight: 18.6 kDa

Tag: C-terminal His-tag

Uses: For in vitro research use only. Not for human in vivo or therapeutic use.

Relevance: Birch trees (Betula), are found in the temperate climate zone of the northern hemisphere and release large amounts of pollen during spring, a major cause of allergic rhinitis in Europe1. A 17 kDa protein called Bet v 1-A2 is the major allergen of birch pollen3. It has been shown that in birch-rich areas of Scandinavia, >95% of birch pollen allergic patients have immunoglobulin E (IgE)-binding to Bet v 14.  Three regions on the surface of the protein contain cross-reactive B-cell epitopes that provide structural basis for similar allergic symptoms when encountering pollens from related trees such as hazel and alder5.


Purity: 98 % as shown by SDS-PAGE

Figure 1: reducing 4-20% SDS-PAGE analysis of 5 µg Bet v 1-A.

Biological activity: Not tested.

Endotoxin: Not tested.


Formulation: Lyophilized from 50 mM HEPES pH7.5, 100 mM NaCl, 175 mM Trehalose Dihydrate.

Shipping: Recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.

Stability & Storage: See COA for detailed storage instructions. Lyophilized materials are stable for up to twelve months from date of receipt at -70℃.

It is recommended that reconstituted protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Reconstitution: A hardcopy of COA with reconstitution instructions is included with the product.


  1. Caillaud D, Martin S, Segala C, et al. Effects of airborne birch pollen levels on clinical symptoms of seasonal allergic rhinoconjunctivitis. Int Arch Allergy Immunol. 163(1):43–50. (2014)
  3. Ipsen H, Løwenstein H. Isolation and immunochemical characterization of the major allergen of birch pollen (Betula verrucosa) J Allergy Clin Immunol. 72(2):150–159. (1983)
  4. Movérare R, Westritschnig K, Svensson M, et al. Different IgE reactivity profiles in birch pollen sensitive patients from six European populations revealed by recombinant allergens: an imprint of local sensitization. Int Arch Allergy Immunol. 128(4):325–335. (2002)
  5. Gajhede M, Osmark P, Poulsen FM, et al. X-ray and NMR structure of Bet v 1, the origin of birch pollen allergy. Nat Struct Biol. 3(12):1040–1045. (1996)